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目的: 构建COX-2基因启动子荧光素酶报告基因重组质粒pGL3-Basic-COX-2-promoter, 并进行功能鉴定. 方法: 根据已知人的COX-2基因启动子序列设计两端引物, 扩增人基因组DNA中的COX-2启动子. 载体质粒pGL3-Basic和COX-2启动子分别用限制性内切酶Hind Ⅲ和Bgl Ⅱ双酶切, 将COX-2基因启动子插入到pGL3-Basic报告载体中. 构建的pG...
Association between the Polymorphisms of IL-4 Gene Promoter (-590C>T),IL-13 Coding Region (R130Q) and IL-16 Gene Promoter (-295T>C) and Allergic Asthma
Allergic asthma Interleukin-4 Interleukin-13 Interleukin-16
2010/4/2
Allergic asthma is a multifactorial disease, influenced by genetic and environmental factors. Recent family-based studies have revealed evidence for linkage of human chromosomes 5q31-33, 12q15-24, 11q...
Association between the Polymorphism of TGF-β1 Gene Promoter (-509C>T) and Idiopathic Chronic Urticaria
Polymorphism TGF-1 gene Urticaria
2010/2/1
Idiopathic Chronic Urticaria (ICU), the most common form (70-80%) of chronic urticaria is supposed to have immune basis causes. It is speculated that the promoter polymorphism of TGF-β1 gene may be in...
Lack of Association between Atopic Dermatitis and -401A/G Polymorphism in the Promoter Region of the RANTES Gene in Japanese
atopic dermatitis IgE polymorphism RANTES
2010/11/22
Background: Regulated on activation, normal T expressed and secreted (RANTES) has been known to be deeply involved in the pathophysiology of atopic dermatitis (AD). To examine whether a -401A/G polymo...