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Cloning and sequencing of the complicated rDNA gene family of Bos taurus
GC-rich repetitive sequences PCR rDNA rRNA Bos Taurus
2015/6/2
The rDNA genes coding for ribosomal RNA (rRNA) in animals are repeat sequences with high GC content and complicated structure. Based on the sequences of human ribosomal DNA repeat unit and transcripti...
以1日龄纯系的优质肉鸡A系(♂，A组)和惠阳胡须鸡(♂，B组)为试验动物，研究比较两纯系鸡28日龄回肠与盲肠黏膜菌群的组成及多样性。于第28日龄分别从2组中采集鸡2个肠段并提取黏膜细菌基因组DNA，应用16S rDNA基因序列技术，建立2个肠段黏膜细菌16S rDNA V3区的随机克隆文库。回肠文库分析发现，A组文库有86个序列共产生6个OTUs（Operational taxonomic uni...