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Interrelationships between the content of oxidative markers,antioxidative status,and somatic cell count in cow’s milk
milk mastitis total antioxidant status malondialdehyde protein oxidation
2016/11/11
Bovine mastitis is a major disease affecting dairy cattle worldwide. The milk collected from cows with different type of inflammation, including mastitis, is characterized by an increased number of so...
Identification of missense mutations in the PCP4 and CD109 genes to validate the effect of neutral genetic markers
immune response local sheep genomics genome-wide association
2016/8/1
The marker-assisted selection exploits anonymous genetic markers that have been associated with measurable differences on complex traits. Because it is based on the linkage disequilibrium (LD) between...
Application of mtDNA markers for European huchen (Hucho hucho Linnaeus, 1758) management in Poland
Salmonidae broodstocks conservation genetic structure mtDNA
2015/12/17
Four broodstocks of European huchen (Hucho hucho) from Poland, Germany, Slovakia, and Ukraine were investigated using five selected fragments of mtDNA. The mitochondrial DNA sequence analysis was succ...
Significance of clinical variables and selected biochemical markers in predicting the outcome of bovine anaplasmosis
Anaplasma marginale cytokine profile oxidative stress prognosis cattle
2015/7/28
The present study was aimed at evaluating the usefulness of selected inflammatory and oxidative stress markers in predicting the clinical outcome of cattle infected with Anaplasma (A). marginale. The ...
Effects of avilamycin and essential oils on mRNA expression of apoptotic and inflammatory markers and gut morphology of piglets
piglets essential oils avilamycin gene expression gut morphology
2015/6/5
In this study 120 piglets were allotted to 3 dietary treatments, negative control group, one group receiving a blend of essential oils (EO) derived from oregano, anise and citrus peels (40 mg/kg diet)...
Genetic diversity of five Chinese goat breeds assessed by microsatellite markers
goat microsatellites biodiversity
2015/6/5
The genetic diversity was studied using microsatellite DNA markers in Laoshan dairy goat (LS), Xinong Saanen dairy goat (SN), Guanzhong dairy goat (GZ), Banjiao goat (BJ) and Guizhou white goat (GW). ...
The use of inflammatory markers as a prognostic aid for traumatic reticuloperitonitis in water buffalo (Bubalus bubalis)
cytokine profile prognosis traumatic reticulo-peritonitis water buffalo
2015/4/17
The present study was conducted to evaluate the prognostic significance of selected inflammatory markers for prediction of clinical outcomes of traumatic reticuloperitonitis (TRP) in water buffalo (Bu...
Lactulose: effect on apoptotic- and immunological-markers in the gastro-intestinal tract of pre-ruminant calves
intestine morphology health
2015/3/13
The study was conducted to elucidate the effects of orally administered lactulose in combination with Enterococcus faecium on immune response of the intestinal tract in pre-ruminant calves. The mRNA e...
Development and Mapping of Microsatellite Markers Derived from cDNA in Japanese Quail (Coturnix japonica)
comparative mapping functional gene Japanese quail linkage map microsatellite
2009/7/31
The objective of this work was to develop polymorphic microsatellite markers derived from Japanese quail embryonic and cardiac cDNA libraries and to locate these markers on Kobe-NIBS Japanese quail (K...
猪微卫星标记多重PCR扩增组合Amplification of Pig Microsatellite Markers Using Multiplex PCR
猪 微卫星 多重PCR
2008/1/10
摘要采用多重PCR的方法，以快速扩增微卫星标记和节约试剂为目标，对其反应条件进行优化后，获得了46个扩增效果理想的微卫星标记多重PCR组合，其中30个为二重PCR，16个为三重PCR。实验结果表明，这些多重PCP反应的引物浓度为0.06~0.3μmol/L， Mg2+的浓度变化范围为1.5~3.0mmol/L,采用的PCR缓冲液的倍数为1.0、1.2、1.4或1.6，每个PCR反应聚合酶的用量在0...
不同猪种肠毒素大肠杆菌（ETEC）F4受体微卫星标记遗传差异性研究The Genetic Variation of Two Microsatellite Markers of Escherichia coli F4(K88) Receptor in Different Swine Breeds
ETEC 微卫星 基因杂合度 Shannon信息指数 遗传标记
2008/1/10
摘要采用13号染色体上与K88ab和K88ac受体基因连锁的2对引物（S0223和S0068），研究沙子岭猪和大约克猪的遗传差异性。结果表明，2个猪种在2个基因座均存在多态性，其基因杂合度和Shannon信息指数存在很大差异，而中外猪种的K88ab和K88ac受体基因也存在遗传差异，这2对引物可望作为K88ab和K88ac受体基因的遗传标记。Abstract: The genetic variat...
利用微卫星标记进行凉山半细毛羊亲权鉴定的研究Microsatellite Markers for Paternity Testing of Liangshan Semi-fine Wool Sheep
凉山半细毛羊 微卫星标记 多重PCR 亲权鉴定
2008/1/6
摘要
经多重PCR扩增20个微卫星标记,对凉山半细毛羊进行亲权鉴定。荧光标记检测扩增产物，并用Cervus 2.0分析。结果表明：当双亲未知时，累积排除概率为0.998666 ，在父系半同胞基础上进行母子亲权鉴定，累积排除概率为0.999994，置信度为95%，最终从8个候选父亲中找出215个后裔的父亲，又从147个候选母亲中找出它们的母亲，构建了一个混合家系系谱，为进行连锁分析、QTL定位...