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High Efficiency Plant Regeneration and Transformation of Watermelon (Citrulus lanatus cv. Giza1)
In vitro Watermelon Kanamycin Transformation Agrobacterium tumefaciens
2010/11/15
Transformation of watermelon using Agrobacterium tumefacien was studied. Plantlets
regeneration were achieved in callus culture of cotyledon and leaf segments from 5-6 days old in vitro grown seedlin...
Agrobacterium-Mediated Transformation in Liliaceous Ornamental Plants
Faculty of Agriculture,Niigata University,Japan
2009/9/10
Studies on Agrobacterium-mediated transformation in 3 Liliaceous ornamental plants, Lilium formosanum, Agapanthus praecox ssp. orientalis and Muscari armeniacum, were described. Three different strain...
Antibiotics for Agrobacterium Elimination in Apple Transformation
cefotaxime Malus × domestica Borkh. meropenem shoot regeneration
2010/11/23
We investigated several antibiotics including carbenicillin (CBPC), clavulanic acid/amoxicillin (CVA/AMPC), cefotaxime (CTX), meropenem (MEPM), vancomycin (VCM) and doxycycline (DOXY) for Agrobacteriu...
Agrobacterium tumefaciens mediated transformation and regeneration of ginger（Zingiber officinale Rosc）
Genetic transformation b-glucuronidase GUS expression
2009/2/19
The absence of seed set in ginger makes conventional breeding methods inapplicable warranting genetic modification through biotechnological means. Agrobacterium tumefaciens strain EHA105/p35SGUSIn...
Genetic Transformation of Citrus paradisi with Antisense and Untranslatable RNA-dependent RNA Polymerase Genes of Citrus tristeza closterovirus
Citrus tristeza virus replicase and RNA-mediated resistance plant transformation RNA-dependent RNA polymerase
2009/1/12
Protein and RNA-mediated forms of pathogen-derived resistance (PDR) have been developed against many viruses in different plants. However, no resistance has been reported against Citrus tristeza viru...
灵芝原生质体的分离及其转化Preparation and Regeneration of the Protoplasts from Ganoderma lucidum and Their Transformation
灵芝 原生质体 再生 转化
2007/12/7
摘要对灵芝(赤芝)进行了原生质体的形成和再生实验,研究了渗透压稳定剂、菌龄、酶解条件对原生质体分离的影响,以及渗透压稳定剂、培养基对原生质体再生的影响;另外还尝试用电穿孔法在灵芝原生质体中进行报告基因(reportgene)β-gal的转化,结果β-gal在菌丝体中有表达。
Abstract:The experiments of protoplasts preparation from Ling...