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Identification of Genes Conferring Resistance to Viral Diseases of Barley Using Multiplex PCR
BaYMV BaMMV BaYMV-2 BYDV Hordeum resistance genes
2016/4/5
This paper describes a rapid and cost-effective assay for identifying economically important barley resistance
genes based on multiplex PCR. A newly created reaction mixture is used for detecting th...
Mycobacterium avium subsp. avium in domestic pigeons (Columba livia f. domestica) diagnosed by direct conventional multiplex PCR: a case report
avian tuberculosis mycobacteriosis zoonosis
2015/4/8
We report three pigeons euthanized in a small household breeding facility, where there was suspicion of an avian tuberculosis outbreak. For rapid identification of Mycobacterium avium subsp. avium dir...
Development of the Multiplex PCR Detection Kit for Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7
foodborne pathogens rapid detection rapid screening
2011/2/24
This review describes the development of the multiplex PCR detection kit for Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7 in food samples. To develop a detection assay, our re...
Multiplex PCR assay to detect rust resistance genes Lr26 and Lr37 in wheat
leaf rust Lr26 Lr37 multiplex PCR wheat
2014/3/10
Multiplex PCR was developed and optimized for simultaneous detection of wheat leaf rust resistance genes Lr26 and Lr37. The presence of the genes was analyzed in 21 winter wheat cultivars registered i...
Identification of New Cry Genes in Bacillus thuringiensis Isolates by Multiplex PCR and RFLP
Bacillus thuringiensis gene characterization biological control multiplex PCR Cry toxins
2010/11/12
Proteins produced by the bacteria Bacillus thuringiensis are widely used for pest control in
agriculture and reforestation. Those proteins are produced by genes known as cry genes. Some members of th...
猪微卫星标记多重PCR扩增组合Amplification of Pig Microsatellite Markers Using Multiplex PCR
猪 微卫星 多重PCR
2008/1/10
摘要采用多重PCR的方法，以快速扩增微卫星标记和节约试剂为目标，对其反应条件进行优化后，获得了46个扩增效果理想的微卫星标记多重PCR组合，其中30个为二重PCR，16个为三重PCR。实验结果表明，这些多重PCP反应的引物浓度为0.06~0.3μmol/L， Mg2+的浓度变化范围为1.5~3.0mmol/L,采用的PCR缓冲液的倍数为1.0、1.2、1.4或1.6，每个PCR反应聚合酶的用量在0...
应用多重PCR和基因扫描技术对五指山猪13个家系32个微卫星基因座的遗传分析Genetic Analysis of 32 Microsatellite Loci in 13 Families of Wuzhishan Pig by Multiplex PCR and Gene scaning Technique
五指山猪 微卫星 基因扫描 遗传多样性
2008/1/6
摘要
五指山猪是我国著名的珍稀畜种,体型小、性成熟早、遗传稳定、近交程度高,可用于医学、药学和生物材料等方面。作者利用多重PCR和基因扫描技术对五指山猪13个家系的32个微卫星基因座进行遗传检测。统计各家系的等位基因组成,计算各家系的平均杂合度和多态信息含量(PIC)；结果,各家系32基因座的平均等位基因数为13.66个,平均PIC为0.731,平均杂合度为0.559。表明海南五指山猪具有丰富的...