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Purification and Characterization of Novel Nucleases from a Thermophilic Fungus
Thermophilic fungi DNase RNase Affinity purification
2014/10/22
A thermophilic fungus was isolated from composted horse manure. The organism was as a Chaetomium sp. by sequencing the highly conserved ITS region of the fungus and comparing to known regions in a gen...
Purification and Characterization of Antioxidant Peptide from Sunflower Protein Hydrolysate
antioxidant peptide sunflower protein hydrolysate purification and characterization
2011/2/24
Sunflower proteins were hydrolyzed with Flavourzyme for the production of antioxidant
peptide. DEAE-Sepharose Fast Flow, Sephadex G-25 gel filtration chromatography
and reversed-phase HPLC were cons...
Purification and Characterization of a Bacteriocin Produced by Lactobacillus lactis Isolated from Marine Environment
Antagonistic activity bacteriocin indicator organisms lactic acid bacteria
2010/9/30
Bacteriocin producing Lactobacillus lactis strain isolated from marine environment, showed broad range of antibacterial activity against some major food borne pathogens. Maximum bacteriocin production...
Purification and Characterization of Three Alkaline Endopolygalacturonases from a Newly Isolated Bacillus gibsonii
alkaline endopolygalacturonases Bacillus gibsonii purification
2009/11/13
A newly isolated Bacillus gibsonii, designated as S-2 (CGMCC1215), was cultivated for production of alkaline pectinases utilizing sugar beet pulp as growth substrate. Purification of three alkaline en...
Separation, Purification and Characterization of Three Endo-polygalacturonases from a Newly Isolated Penicillum oxalicum
characterization Penicillum oxalicum endo-polygalacturonase
2009/11/13
Three endo-polygalacturonases (endoPGs) from a newly isolated Penicillum oxalicum (CGMCC 0907) capable of utilizing waste biomass as growth substrate were separated and purified to homogeneity by ultr...
Purification and Characterization of Extracellular Cysteine Protease Inhibitor from Chlorella sp.
green algae Chlorella cysteine protease inhibitor
2009/7/8
An extracellular cysteine protease inhibitor (ECPI) from Chlorella sp. was purified to homogeneity by DEAE-Cellulose column chromatography and Sephacryl S-300 column chromatography. The molecular mass...
Purification and Characterization of Phospholipase D from Cabbage Leaves
phospholipase D cabbage N-terminal amino acid sequence
2009/7/8
Phospholipase D (PLD) was purified from cabbage leaves. The molecular weight of purified PLD was estimated as approximately 73 and 87 kDa by gel filtration using Superdex 200 HR column and, sodium dod...
Purification and Characterization of Feruloyl Esterase from Aspergillus awamori
Aspergillus awamori feruloyl carbohydrate ester feruloyl esterase
2009/7/7
A. awamori feruloly esterases, termed FAE-a and FAE-b, were purified to homogeneity by ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, DEAE-Toyopearl 650 column chromatograph...
Purification and Characterization of Intracellular Cysteine Protease Inhibitor from Chlorella sp.
green algae Chlorella cysteine protease inhibitor
2009/7/7
An intracellular cysteine protease inhibitor (ICPI) from Chlorella sp. was purified to homogeneity judging from polyacrylamide gel electrophoresis. The purified inhibitor had a molecular mass of 410kD...
Purification and Characterization of Phytase from Bran of Triticum aestivum L. cv. Nourin #61
phytase phytic acid wheat bran
2009/7/7
Two phytase isozymes (PHY1 and PHY2) were purified homogeneously from bran of Triticum aestivum L. cv. Nourin #61 by (NH4)2SO4 fractionation, methanol fractionation, Sephacryl S-200 HR gel filtration ...
Purification and Characterization of Glutamate Decarboxylase from Aspergillus oryzae
glutamate decarboxylase Aspergillus oryzae purification
2009/6/17
We purified glutamate decarboxylase (GAD) [EC4.1.1.15] from Aspergillus oryzae and characterized its biochemical and kinetic properties. GAD was purified by ammonium sulfate at 30–70% saturation and c...
Purification and Characterization of Wheat（Triticum aestivum）Polyphenol Oxidase
wheat polyphenol oxidase purification
2009/6/10
Polyphenol oxidase was purified to homogeneity from Japanese wheat (Triticum aestivum cv. Tohoku 198) bran, using 4-methylcatechol as a substrate. The enzyme was purified 1670-fold with a recovery of ...
Purification and Characterization of an Extracellular Proteinase Having Milk-Clotting Activity from Enterococcus faecalis TUA2495L
milk-clotting enzyme extracellular proteinase Enterococcus faecalis
2009/5/31
Lactic acid bacteria (157 stock cultures) were screened for their ability to produce extracellular proteinase with milk-clotting activity. A strain identified as Enterococcus faecalis TUA2495L showed ...
Purification and Characterization of Polyphenol Oxidase from Edible Yam（Dioscorea opposita Thunb.）
polyphenol oxidase edible yam dopamine oxidase
2009/5/31
Soluble polyphenol oxidase of edible yam (Dioscorea opposita Thunb.) was purified approximately 203-fold with a recovery rate of 15% by ammonium sulfate fractionation, ion exchange chromatography, hyd...
Purification and Characterization of Tannin Acyl Hydrolase from Aspergillus niger ATCC 16620
tannase fermentation gel filtration purification
2009/3/16
Tannin acyl hydrolase produced extracellularly by the fungal strain Aspergillus niger ATTC 16620 in solid state fermentation was purified from the cell free culture broth by ammonium sulphate fraction...